ZebraVesselCode: Lymphatic endothelial cell explorer
Version 2 (latest version)
Record description
- Title
- ZebraVesselCode: Lymphatic endothelial cell explorer
- DOI
- https://doi.org/10.82595/scilifelab.a3khw-87p96
- Description
- Cell proliferation underpins organ formation, yet how spatio-temporal expansion of lymphatic endothelial cells (LECs) is controlled remains unclear. In this study, we used single-cell transcriptomic analysis at four different timepoints to determine genes that are enriched in proliferating LECs. Single-cell RNA-seq data were performed on LECs and venous endothelial cells (VECs) isolated by fluorescence-activated cell sorting of double-positive Tg(fli1a:nEGFP)y7;Tg(-5.2lyve1b:DsRed2)nz101 cells from zebrafish embryos at 36 hours post-fertilisation (hpf), 48 hpf, 5 days post-fertilisation, and 7 dpf. These timepoints represent the immature LEC progenitors to differentiated functional LECs lining the lumen of lymphatic vessels. This analysis identified genes forming a unique LEC proliferation code activated during bursts of LEC proliferation. We further functionally validated that lbr, fkbp1ab, and armc1l, LEC proliferation code components, play a role in lymphatic progenitor expansion, and that the code is conserved in mammals. This study is currently available as a preprint: Marleen Gloger, Marle Kraft, Di Peng et al. Lineage-enriched cell proliferation code underlies spatio-temporal regulation of cell cycle dynamics in lymphatic vessels, 11 March 2026, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-5333191/v1]
- Subjects and keywords
- cell differentiation cell proliferation lymphatic vessels single-cell analysis zebrafish
- URL
- https://lymphatic-proliferation-zebrafish.serve.scilifelab.se
- Source code
- https://github.com/LymphaticsLab/LEC-proliferation
- Docker image
- ghcr.io/lymphaticslab/lecprol_shinyapp:v1.0
- Date submitted
- 2026-06-23 13:34
- Date updated
- 2026-06-24 14:03
- Date available
- 2026-06-23 13:34
- Language
- English
- Funding
-
Knut and Alice Wallenberg Foundation (Defining the role of epigenomics in lymphatic vessel development, homeostasis and disease)Swedish Research Council (Heterogeneity in progenitor cells during lymphatic vascular development)
Creators
Koltowska, Kaska
Versions
Version 2:
https://doi.org/10.82595/scilifelab.a3khw-87p96 current
https://doi.org/10.82595/scilifelab.a3khw-87p96 current
